Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neuropeptide-processing carboxypeptidases.

Suwen Wei1, Yun Feng, Elena Kalinina

  • 1Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.

Life Sciences
|June 13, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hypercontractile phenotype at rest in chronic coronary syndromes predicts impaired functional reserve and increased mortality.

ESC heart failure·2026
Same author

Editorial for the Special Issue on Advanced Thin Films: Design, Fabrication, and Applications, 2nd Edition.

Micromachines·2026
Same author

Editorial for the Special Issue on Fabrication, Characterization and Application of Organic/Inorganic Film Membranes and Advanced Materials (Volume II).

Membranes·2026
Same author

Editorial for the Special Issue on Fabrication, Characterization and Application of Organic/Inorganic Film Membranes and Advanced Materials (Volume III).

Membranes·2026
Same author

Moderate ischemic mitral regurgitation at rest is associated with multiple functional vulnerabilities during exercise stress echocardiography.

The international journal of cardiovascular imaging·2026
Same author

Male Stress Is Associated with Ovarian and Endometrial Responses in ICSI Cycles: Is Seminal Plasma the Linchpin?

International journal of molecular sciences·2026

Researchers identified CPA-5, a novel enzyme, which may process neuropeptides like beta-endorphin at non-basic amino acid sites. This finding sheds light on alternative neuropeptide processing pathways in the brain and pituitary gland.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Molecular Biology

Background:

  • Neuropeptides are typically processed from precursor proteins via cleavage at basic amino acid sites by enzymes like prohormone convertases.
  • Several neuropeptides undergo cleavage at non-basic sites, but the responsible enzymes remain largely unidentified.

Purpose of the Study:

  • To investigate the role of a newly discovered human metallocarboxypeptidase gene, CPA-5, in neuropeptide processing.
  • To determine if CPA-5 protein distribution in the pituitary is consistent with its involvement in non-basic cleavage of proopiomelanocortin (POMC)-derived peptides.

Main Methods:

  • Bioinformatic search for novel metallocarboxypeptidase family members.
  • Analysis of CPA-5 mRNA expression in human brain, pituitary, and testis.

Related Experiment Videos

  • Immunocytochemistry to examine CPA-5 protein distribution in the pituitary and AtT-20 corticotroph cells.

    • Main Results:

    • A novel human gene, CPA-5, was identified with specificity for C-terminal hydrophobic amino acids and expression in the brain, pituitary, and testis.
    • CPA-5 protein was detected in the pituitary's neurointermediate lobe and scattered anterior lobe cells.
    • In AtT-20 cells, CPA-5 exhibited a perinuclear distribution.

    Conclusions:

    • The expression and localization patterns of CPA-5 in the pituitary are consistent with a role in the intracellular processing of POMC-derived peptides.
    • CPA-5 may be involved in the non-basic cleavage pathway for neuropeptides such as beta-endorphin and adrenocorticotropin.