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Atp Binding Cassette Transporter Gene Expression In Rat Liver Progenitor Cells.

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Atp Binding Cassette Transporter Gene Expression In Rat Liver Progenitor Cells.

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ATP binding cassette transporter gene expression in rat liver progenitor cells.

J E Ros¹, T A D Roskams, M Geuken

¹Groningen University Institute for Drug Exploration (GUIDE), Centre for the Study of Liver, Digestive, and Metabolic Diseases, University Hospital Groningen, 9700 RB Groningen, the Netherlands.

|June 13, 2003

View abstract on PubMed

Summary

This summary is machine-generated.

Hepatic progenitor cells (HPCs) express high levels of multidrug resistance-associated protein 1 (MRP1) and MRP3. These ATP binding cassette (ABC) transporters may protect HPCs during severe liver damage.

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Area of Science:

Hepatology
Cell Biology
Molecular Biology

Background:

Liver regeneration relies on hepatic progenitor cells (HPCs) that must survive toxic liver conditions.
ATP binding cassette (ABC) transporters are efflux pumps that may protect cells.

Purpose of the Study:

To determine the ABC transporter phenotype of hepatic progenitor cells (HPCs).

Main Methods:

HPC activation was induced in rats using 2-acetylaminofluorene (2-AAF) and partial hepatectomy (PHx).
ABC transporter gene expression was analyzed using RT-PCR in isolated cells and immunohistochemistry in liver samples.
Efflux activity of ABC transporters was assessed in cultured progenitor cells via flow cytometry.

Main Results:

Rats treated with 2-AAF/PHx showed increased mRNA levels for Mdr1b, Mrp1, and Mrp3.

Immunohistochemistry revealed Mrp1 and Mrp3 expression in progenitor cells and Mdr1b in hepatocytes.

Isolated HPCs and cultured progenitor cells predominantly expressed Mrp1 and Mrp3 mRNA, with minimal expression of hepatocyte-specific transporters.

Conclusions:

Hepatic progenitor cells (HPCs) exhibit high expression of active Mrp1 and Mrp3.
These transporters likely play a cytoprotective role for HPCs in hepatotoxic environments.