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A sensitive and practical competitive radioassay for plasma biotin
Catherine Harthé1, Bruno Claustrat
1Service de Radiopharmacie et de Radioanalyse, Centre de Médecine Nucléaire, 59 Blvd Pinel, 69394 Lyon Cedex 03, France.
Annals of Clinical Biochemistry
|June 14, 2003
Summary
A new assay accurately measures biotin levels in human plasma, aiding in the crucial diagnosis of biotin deficiency. This method is sensitive, requiring only a small sample volume and rapid processing for efficient screening.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Nutritional Science
Background:
- Biotin is an essential water-soluble vitamin vital for carboxylase-mediated metabolic reactions.
- Accurate determination of biotin status is critical for diagnosing biotin deficiency.
Purpose of the Study:
- To develop and validate a novel assay for direct biotin measurement in human plasma.
- To assess the assay's suitability for screening biotin deficiency.
Main Methods:
- A one-step, solid-phase protein-binding assay utilizing [(125)I]iodostreptavidin as a tracer.
- Competitive binding principle where biotin in samples occupies sites on the tracer.
Main Results:
- The assay demonstrated a detection limit of 100 pmol/L for plasma biotin.
- Requires only 100 microL of plasma and can be completed within 6 hours.
- Intra-assay (3.5%) and inter-assay (10%) coefficients of variation were reported.
Conclusions:
- The developed assay is suitable for direct and sensitive measurement of biotin in human plasma.
- The assay is robust and sensitive enough for effective screening of biotin deficiency.