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Related Experiment Videos

AKAP7gamma is a nuclear RI-binding AKAP.

Rebecca L Brown1, Shelley L August, Carmen J Williams

  • 1Center for Research on Reproduction and Women's Health, Department of Obstetrics and Gynecology, University of Pennsylvania, Rm. 1312 BRB II/III, 421 Curie Blvd., Philadelphia, PA 19104-6142, USA.

Biochemical and Biophysical Research Communications
|June 14, 2003
PubMed
Summary

A-kinase anchor proteins (AKAPs) regulate protein kinase A (PKA) localization. AKAP7gamma anchors PKA in the nucleus, potentially regulating gene transcription in oocytes and somatic cells.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein Kinase A (PKA) activity is crucial for mammalian oocyte development.
  • Spatial regulation of PKA is mediated by A-kinase anchor proteins (AKAPs).

Purpose of the Study:

  • To identify AKAPs involved in oocyte development.
  • To investigate the role of AKAP7gamma in PKA localization and function.

Main Methods:

  • AKAP screening in oocytes
  • Yeast two-hybrid analysis
  • Co-immunoprecipitation studies
  • HEK 293 cell transfection and localization studies

Main Results:

  • AKAP7gamma was identified as an AKAP in oocytes.

Related Experiment Videos

  • AKAP7gamma binds the type I PKA regulatory subunit (RI).
  • AKAP7gamma contains a nuclear localization signal and is found in both nuclear and cytoplasmic compartments of oocytes.

    • Conclusions:

    • AKAP7gamma is the first identified nuclear AKAP that binds RI.
    • AKAP7gamma may position PKA via RI and/or RII to regulate gene transcription.
    • AKAP7gamma plays a role in PKA-mediated gene transcription in both somatic cells and oocytes.