Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Motilin-bicelle interactions: membrane position and translational diffusion.

August Andersson1, Lena Mäler

  • 1Department of Biochemistry and Biophysics, Arrhenius Laboratory, Stockholm University, 10691, Stockholm, Sweden.

FEBS Letters
|June 14, 2003
PubMed
Summary

This study reveals how the peptide hormone motilin interacts with bicelles, showing significant binding and insertion into the lipid layers. These findings advance our understanding of peptide-lipid interactions in biological systems.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure and membrane interactions of Arabidopsis thaliana DGD2, a glycosyltransferase in the chloroplast membrane.

The Journal of biological chemistry·2025
Same author

Structural and functional insights into the Pseudomonas aeruginosa glycosyltransferase WaaG and the implications for lipopolysaccharide biosynthesis.

The Journal of biological chemistry·2023
Same author

Lipid- and substrate-induced conformational and dynamic changes in a glycosyltransferase involved in E. coli LPS synthesis revealed by <sup>19</sup>F and <sup>31</sup>P NMR.

Biochimica et biophysica acta. Biomembranes·2023
Same author

Aerosol demasking enhances climate warming over South Asia.

NPJ climate and atmospheric science·2023
Same author

Black carbon aerosols over Indian Ocean have unique source fingerprint and optical characteristics during monsoon season.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Atmospheric Black Carbon Loadings and Sources over Eastern Sub-Saharan Africa Are Governed by the Regional Savanna Fires.

Environmental science & technology·2022

Area of Science:

  • Biochemistry
  • Biophysics
  • Structural Biology

Background:

  • Motilin is a peptide hormone involved in gastrointestinal motility.
  • Bicelles are model membrane systems used to study protein-lipid interactions.
  • Understanding peptide-membrane interactions is crucial for drug development and biological research.

Purpose of the Study:

  • To investigate the binding interaction between the peptide hormone motilin and bicelles.
  • To determine the location and orientation of motilin within the bicelle structure.
  • To elucidate the effect of motilin on bicelle properties.

Main Methods:

  • Pulsed field gradient-nuclear magnetic resonance (PFG-NMR) spectroscopy was employed to measure diffusion coefficients.
  • Paramagnetic probes were utilized to assess the positioning of motilin within the bicelles.

Related Experiment Videos

  • Diffusion coefficients of motilin, phospholipids, and tetramethylsilane were analyzed.

    • Main Results:

    • Approximately 90% of motilin bound to acidic bicelles and 84% to neutral bicelles.
    • Motilin binding led to a reduction in the apparent size of the bicelles.
    • Paramagnetic data indicated insertion of motilin's N-terminus into the bicelle and its helix within the head-group layer.

    Conclusions:

    • Motilin exhibits strong binding affinity for both acidic and neutral bicelles.
    • The interaction influences bicelle structure, causing a decrease in apparent size.
    • Motilin's structure is partially inserted into the bicelle, with specific regions interacting with different parts of the lipid bilayer.