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Rat lymphoid cell--derived histamine and 5-hydroxytryptamine releasing factor
J Wyczółkowska1, Z Sułowska, J Dastych
1Department of Biogenic Amines of Polish Academy of Sciences, Lódź.
Summary
Lymphoid cells produce histamine releasing factor (HRF) that triggers mast cell degranulation. Nippostrongylus brasiliensis infection enhances this factor
Area of Science:
- Immunology
- Cell Biology
- Parasitology
Background:
- Histamine releasing factor (HRF) plays a role in allergic responses.
- Understanding HRF production by lymphoid cells is crucial for immune studies.
Purpose of the Study:
- To investigate the in vitro production of HRF by rat lymphoid cells.
- To determine the effect of Nippostrongylus brasiliensis infection on HRF production.
- To characterize the properties of rat HRF.
Main Methods:
- Culturing rat spleen cells and thymocytes with and without PHA.
- Testing cell-free supernatants for histamine and 5-hydroxytryptamine releasing activity on mast cells.
- Determining HRF molecular weight and stability using gel chromatography.
Main Results:
- PHA-stimulated normal rat spleen cells and thymocytes produced HRF.
- N. brasiliensis infection potentiated HRF production by lymphoid cells.
- Pleural mast cells were more sensitive to HRF than peritoneal mast cells.
- Rat HRF is a heat-stable protein (50-70 kDa) inducing rapid histamine release.
Conclusions:
- Rat lymphoid cells, particularly when stimulated, produce HRF.
- Parasitic infection can modulate HRF production.
- HRF is a potent mediator of rapid mast cell degranulation.