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The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species

E S Visser1, T C McGuire, G H Palmer

  • 1Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.

Infection and Immunity
|December 11, 1992
PubMed

Insights

Anaplasma marginale outer membrane protein 5 (MSP-5) is a conserved antigen across Anaplasma species. Antibodies to MSP-5 can identify persistently infected cattle, aiding in disease diagnosis.

Area of Science:

  • Veterinary Immunology
  • Molecular Biology
  • Parasitology

Background:

  • Immunization with Anaplasma marginale outer membranes confers immunity against clinical disease.
  • Immunity correlates with antibody titers to outer membrane proteins, including a 19-kDa protein.

Purpose of the Study:

  • To characterize the 19-kDa outer membrane protein, designated major surface protein 5 (MSP-5).
  • To evaluate MSP-5 as a potential diagnostic antigen for Anaplasma infections.

Main Methods:

  • Gene sequencing to identify the MSP-5 encoding gene.
  • Immunoblotting with monoclonal antibody ANAF16C1 to assess molecular mass.
  • Competitive inhibition assays using recombinant MSP-5 and immune sera.

Main Results:

  • MSP-5 is encoded by a single-copy 633-bp gene.
  • The molecular mass of MSP-5 is conserved across Anaplasma marginale, A. centrale, and A. ovis.
  • Antibodies to the epitope defined by ANAF16C1 are present in post-infection sera.

Conclusions:

  • MSP-5 is a conserved Anaplasma antigen.
  • The epitope defined by ANAF16C1 is a potential diagnostic marker for persistent Anaplasma infections in cattle.

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