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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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Published on: April 3, 2014

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized

T Urabe1, K Sano, M Tanno

  • 1Department of Microbiology, Osaka Medical College, Japan.

Journal of Virological Methods
|November 1, 1992
PubMed

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...