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Related Experiment Videos

Developmental changes in nerve growth factor level in rat serum.

K Murase1, R Takeuchi, E Iwata

  • 1Department of Molecular Biology, Gifu Pharmaceutical University, Japan.

Journal of Neuroscience Research
|October 1, 1992
PubMed
Summary
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Serum nerve growth factor (NGF) levels were reliably measured using a guanidine hydrochloride method. NGF levels increase during early development and pregnancy, suggesting a role in peripheral nervous system establishment.

Area of Science:

  • Neuroscience
  • Biochemistry

Background:

  • Nerve growth factor (NGF) in serum forms a complex with alpha 2-macroglobulin (alpha 2M).
  • This complexation inhibits NGF's immunoreactivity, complicating accurate serum level measurement.
  • Liberating NGF from the complex and preventing its re-formation is crucial for reliable quantification.

Purpose of the Study:

  • To develop a reliable method for measuring serum NGF levels in rats.
  • To investigate developmental and pregnancy-related changes in serum NGF.

Main Methods:

  • Serum pretreatment with 1 M guanidine hydrochloride to dissociate the NGF-alpha 2M complex.
  • Enzyme immunoassay (EIA) performed in the presence of guanidine hydrochloride to prevent complex re-formation.
  • Monitoring serum NGF levels in rats across different developmental stages and during pregnancy.

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Main Results:

  • A reliable method for serum NGF determination was established using guanidine hydrochloride pretreatment and EIA.
  • Serum NGF levels increased from prenatal day 2 to postnatal day 5 in rats.
  • A slight decrease was observed at postnatal week 3, with levels stabilizing in adulthood.
  • Pregnant rats exhibited a threefold to fivefold increase in serum NGF before birth, followed by a rapid decline.

Conclusions:

  • Serum NGF levels can be accurately measured using a guanidine hydrochloride-assisted EIA method.
  • Serum NGF levels exhibit dynamic changes during development and pregnancy.
  • These fluctuations suggest that serum NGF plays a role in the establishment of the peripheral nervous system.