Reducing colonization of Salmonella Enteritidis in chicken by targeting outer membrane proteins
M I Khan1, A A Fadl, K S Venkitanarayanan
1Department of Pathobiology, University of Connecticut, Storrs, CT 06269, USA. mazhar.khan@uconn.edu
Aims:
To evaluate the ability of Salmonella enterica ser. Enteritidis outer membrane proteins (OMPs) of 75.6 and 82.3 kDa to inhibit or reduce in vivo colonization of S. Enteritidis on intestinal mucosa in chickens.
Methods And Results:
Nine-week-old specific-pathogen-free chickens were subcutaneously immunized with 75.6 or 82.3 kDa protein, and challenged with a virulent strain of S. Enteritidis. Chickens were killed, and portions of small intestine and caecum were removed at necropsy. The population of S. Enteritidis attached to chicken intestinal mucosa was determined. The population of S. Enteritidis recovered from the small intestine and caecum of chickens immunized with 75.6 or 82.3 kDa protein was significantly (P < 0.05) lower than that recovered from the control birds.
Conclusions:
Salmonella Enteritidis OMPs 75.6 kDa and 82.3 kDa were effective in reducing colonization of S. Enteritidis on intestinal mucosa in chickens.
Significance And Impact Of The Study:
Salmonella Enteritidis OMPs 75.6 or 82.3 kDa could be used as potential vaccines to reduce S. Enteritidis colonization in chickens.
Related Concept Videos
01:23Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
01:28Biological Methods for Microbial Control