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Chapter 12: Human papillomavirus technologies.

Thomas Iftner1, Luisa Lina Villa

  • 1Medical Virology, Section Experimental Virology, University Hospital of Tübingen, Elfriede-Aulhorn Strasse 6, 72076 Tübingen, Germany. tsiftner@med.uni-tuebingen.de

Journal of the National Cancer Institute. Monographs
|June 17, 2003
PubMed
Summary

Current human papillomavirus (HPV) DNA tests include hybrid capture and PCR methods. Novel technologies are needed for large-scale screening, requiring standardized protocols and reference samples for validation.

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Area of Science:

  • Virology
  • Molecular Diagnostics
  • Public Health

Background:

  • Current human papillomavirus (HPV) DNA detection relies on hybrid capture second-generation (HC2) assays and polymerase chain reaction (PCR) protocols.
  • Existing methods are validated in large trials and epidemiological studies but require further development for optimal screening.

Purpose of the Study:

  • To review currently available technologies for HPV detection.
  • To discuss novel technologies and their potential for large-scale HPV screening.
  • To outline ideal characteristics for future HPV testing systems.

Main Methods:

  • Review of existing HPV DNA testing technologies, including HC2 and PCR-based methods.
  • Discussion of emerging technologies for HPV detection and their suitability for high-throughput analysis.

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  • Identification of requirements for future HPV tests, such as multi-type detection, individual type identification, and quantitative viral load measurement.

    • Main Results:

    • Current HPV tests have limitations in detecting multiple types, quantifying viral load, and ease of use for high-throughput screening.
    • Ideal HPV tests should be highly sensitive (detecting at least 10,000 genome copies/sample for high-risk types), specific, reproducible, and automatable.
    • There is an urgent need for reference samples, validated reagents, and standardized protocols to validate current and future HPV test systems.

    Conclusions:

    • Advancements in HPV detection technology are crucial for effective large-scale screening programs.
    • Standardization and validation are essential to ensure the reliability and comparability of different HPV testing platforms.
    • Future HPV tests must balance sensitivity, specificity, and practicality for clinical and epidemiological applications.