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Molecular cloning of equine muscle-type phosphofructokinase cDNA
Tetsuo Sato1, Takuya Itou, Takeo Sakai
1Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Abstract:
The complete coding region sequence of equine muscle-type phosphofructokinase (ePFKM) was obtained from skeletal muscle of a thoroughbred horse. The deduced amino acid sequence of ePFKM showed 97%, 96%, 96%, 96% and 95% identity to canine, human, mouse, rabbit and rat PFKM, respectively. The amino and carboxyl terminal halves of ePFKM presented a structure of tandem repeat, as other mammalian PFKMs. As the amino acid residues constituting various ligand-binding sites were also conserved, it is thought that ePFKM has enzymatic activity similar to PFKM in other mammals.