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Related Experiment Videos

Improved biotransformations on charged PEGA supports.

Alessandra Basso1, Luigi De Martin, Lucia Gardossi

  • 1Dipartimento di Scienze Farmaceutiche, Università degli Studi, Piazzale Europa 1, 34127, Trieste, Italy.

Chemical Communications (Cambridge, England)
|June 18, 2003
PubMed
Summary

Charged polyethylene glycol (PEG) supports exhibit enhanced swelling in water compared to neutral PEG. A new positively charged PEG resin boosts penicillin G amidase (PGA) biotransformation by improving enzyme accessibility.

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Area of Science:

  • Biocatalysis
  • Polymer Chemistry
  • Enzyme Immobilization

Background:

  • Polyethylene glycol (PEG) supports are utilized in enzyme immobilization.
  • Surface charge influences the properties of PEG-based materials in aqueous environments.
  • Penicillin G amidase (PGA) is an industrially relevant enzyme.

Purpose of the Study:

  • To investigate the impact of permanent charges on PEG support swelling properties.
  • To develop and evaluate a novel positively charged PEG resin for enzyme catalysis.
  • To assess the effect of the charged support on penicillin G amidase (PGA) activity.

Main Methods:

  • Synthesis of neutral and permanently charged PEG supports.
  • Characterization of swelling properties in aqueous media.

Related Experiment Videos

  • Immobilization of penicillin G amidase (PGA) onto the developed supports.
  • Evaluation of biocatalytic activity in penicillin G production.

    • Main Results:

    • Permanently charged PEG supports demonstrated superior swelling compared to neutral PEG supports.
    • The novel positively charged PEG resin significantly enhanced PGA-catalyzed biotransformation.
    • Improved accessibility of the negatively charged PGA enzyme to the positively charged support was observed.

    Conclusions:

    • Permanent charges on PEG supports enhance swelling in aqueous media.
    • Positively charged PEG resins are effective for immobilizing negatively charged enzymes like PGA.
    • This approach offers a promising strategy for improving enzyme-catalyzed biotransformations on solid supports.