[Interference of chloramphenicol with some cytoplasmatic enzyme systems]

The activity of alkaline phosphatase was determined in a lot of Wistar white rats which had received during 8 days therapeutical doses of chloramphenicol hemisuccinate, the enzyme being investigated in the circulating granulocytes and the liver. In the neutrophil granulocytes evidence was found of a marked decrease in alkaline phosphatase activity (40% presented a positive activity in the injected animals as against 70% in the controls). In the liver the histochemical test used did not allow for a discrimination between the experimental and control animals. The biochemical test, however, showed depression of alkaline phosphatase activity in the liver of rats receiving chloramphenicol (216.72 muM PO4 mean value as against 267.36 muM PO4).