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[Relationship between aquaporin-4 expression in astrocytes and brain edema caused by glioma]
Yi-zhao Chen1, Ru-xiang Xu, Zong-jun Xu
1Department of Neurosurgery, First Military Medical University, Guangzhou 510282, China. chenyizhao@21cn.com
Objective:
To investigate the relationship between aquaporin-4 (AQP4) water channel expression in astrocytes and brain edema caused by glioma.
Methods:
The changes of water transport in in vitro blood-brain barrier models were examined using high-performance liquid chromatography (HPLC), and the expression level of AQP4 was assayed by semiquantitative RT-PCR.
Results:
The water transport of the in vitro blood-brain barrier model from the luminal side to basolateral side was increased after coculture with glioma cells, which induced significantly decreased expression level of AQP4 in the astrocytes.
Conclusions:
Glioma cells can promote water transport in in vitro blood-brain barrier model from the luminal side to abluminal side, and the brain edema induced by the glioma cells may not necessarily be the result of hyperpermeability of the blood-brain barrier to macromolecules in the plasma. The decreased expression level of AQP4 induced by glioma cells may be a molecular mechanism for neoplastic brain edema in patients with glioma.