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Updated: Sep 17, 2026
Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
Published on: May 15, 2015
[Purification of porcine pulmonary surfactant by CO2 supercritical fluid extraction]
Qian Zhao1, Zhi-chun Feng, Jiang Du
1Department of Pediatrics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Objective:
To develop a supercritical fluid extraction (SFE) method for purifying porcine pulmonary surfactant.
Method:
An orthogonal design with 4 factors and 4 levels was used to optimize the SFE conditions.
Results:
The best extraction conditions were established as the following: pressure 30 MPa, temperature 50 degrees Celsius, dynamic extraction for 4 h with 0.2 ml ethanol as the modifier.
Conclusion:
The new method for purifying porcine pulmonary surfactant by SFE-CO2 can cause drastic reduction of organic solvents, being convenient to use and effective to purify.
