Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reticulocyte quantification by flow cytometry, image analysis, and manual counting.

K J Schimenti1, K Lacerna, A Wamble

  • 1Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106-4901.

Cytometry
|January 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diverse excess GABA modes drive autism and epilepsy-autism comorbidity.

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Ephaptic conduction molding memory engrams.

BMC biology·2025
Same author

A modest proposal to the transplant publik to prevent harm to people with acute myeloid leukaemia in 1st complete remission cured by chemotherapy.

Leukemia·2024
Same author

Possible mechanism of schizophrenia origin by excess GABA and synaptic pruning.

IBRO neuroscience reports·2023
Same author

A possible new cardiac heterogeneity as an arrhythmogenic driver.

Scientific reports·2023
Same author

Percolation and tortuosity in heart-like cells.

Scientific reports·2021

Flow cytometry with thiazole orange offers improved reticulocyte counting precision compared to manual methods. Adjusting thiazole orange concentration enhances sensitivity, making it a more effective tool for reticulocyte enumeration.

Area of Science:

  • Hematology
  • Clinical Pathology
  • Biotechnology

Background:

  • Accurate reticulocyte counting is crucial for assessing bone marrow function, especially in transplant patients.
  • Traditional methods include manual counting and automated analysis of new methylene blue-stained smears.
  • Flow cytometry offers a potential alternative for reticulocyte enumeration.

Purpose of the Study:

  • To compare the sensitivity and precision of reticulocyte counting using flow cytometry with thiazole orange versus manual and automated methods.
  • To optimize thiazole orange concentration and analysis gating for improved reticulocyte detection.

Main Methods:

  • Reticulocyte counts were performed using flow cytometry with thiazole orange and compared to manual and automated new methylene blue staining methods.

Related Experiment Videos

  • Initial comparisons involved 49 clinical samples and 289 bone marrow transplant patient samples.
  • Further studies optimized thiazole orange concentration and analyzed different fractions (40%, 60%, 80%, 100%) of brightest reticulocytes.

    • Main Results:

    • Initial flow cytometry with thiazole orange showed lower sensitivity than new methylene blue (slopes < 1).
    • Increasing thiazole orange concentration and analyzing larger reticulocyte fractions (up to 100%) improved sensitivity, with slopes approaching or exceeding 1.
    • Flow cytometry demonstrated superior precision compared to image analysis and manual counting.

    Conclusions:

    • Optimized flow cytometry with thiazole orange provides a sensitive and precise method for reticulocyte counting.
    • This technique is particularly advantageous for monitoring bone marrow recovery in transplant recipients.
    • Flow cytometry offers a significant advancement over traditional manual and automated counting methods in terms of precision.