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Polymeric FAD used as enzyme-friendly mediator in lactate detection
Mihaela D Leonida1, Daniel T Starczynowski, Riva Waldman
1Fairleigh Dickinson University, SONS, H444 N, Teaneck, NJ 07666, USA. mleonida@fdu.edu
Analytical and Bioanalytical Chemistry
|June 18, 2003
Summary
New lactate biosensors utilize polymerized flavin adenine dinucleotide (FAD) for efficient electron transfer. These sensors, using lactate dehydrogenase (LDH) or lactate oxidase (LOX), demonstrate good sensitivity and stability for potential applications.
Area of Science:
- Electrochemistry
- Biotechnology
- Biosensor technology
Background:
- Lactate detection is crucial in clinical diagnostics and biotechnology.
- Mediators are essential for efficient electron transfer in biosensors.
- Polymerized flavin adenine dinucleotide (FAD) offers a novel approach for electron mediation.
Purpose of the Study:
- To fabricate and characterize novel lactate biosensors.
- To investigate the use of polymerized FAD as an electron transfer mediator.
- To evaluate biosensor performance using different enzyme immobilization strategies.
Main Methods:
- Fabrication of electrodes modified with polymerized FAD.
- Immobilization of lactate dehydrogenase (LDH), lactate oxidase (LOX), or baker's yeast (BY) onto the electrode surface.
- Electrochemical characterization of the developed biosensors.
Main Results:
- The developed lactate biosensors exhibited good sensitivity, linearity, and stability.
- Sensors utilizing purified enzymes (LDH, LOX) showed optimal performance.
- Baker's yeast (BY) immobilized electrodes demonstrated slightly lower sensitivity but offered a viable alternative.
Conclusions:
- Polymerized FAD is an effective mediator for lactate biosensors.
- The fabricated biosensors show promise for various lactate monitoring applications.
- Further research can optimize BY-based sensors for broader utility.