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Rapid fluorimetric assay for primary amine groups in water samples
A Sevillano Cabeza1, Y Moliner Martinez, C Molins Legua
1Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
Analytical and Bioanalytical Chemistry
|June 18, 2003
Summary
This study presents a new method for detecting primary amines in water using solid-phase extraction and derivatization with o-phthaldialdehyde-N-acetylcysteine (OPA-NAC). The optimized technique significantly improves detection limits for environmental water analysis.
Area of Science:
- Analytical Chemistry
- Environmental Science
Background:
- Primary amines are crucial indicators in water quality assessment.
- Accurate quantification of amines in diverse water matrices presents analytical challenges.
Purpose of the Study:
- To develop and optimize a solid-phase extraction (SPE) method for pre-concentrating primary amines in water.
- To validate a derivatization technique using o-phthaldialdehyde-N-acetylcysteine (OPA-NAC) for enhanced fluorescence detection.
- To establish optimal conditions for amine determination in various water samples.
Main Methods:
- Utilized Bond Elut C(18) solid-phase extraction cartridges for analyte pre-concentration.
- Employed derivatization with o-phthaldialdehyde-N-acetylcysteine (OPA-NAC) for sensitive fluorescence detection.
- Optimized buffer pH, reagent concentrations, and elution conditions for maximum efficiency.
Main Results:
- Achieved a tenfold improvement in detection limits compared to solution derivatization alone.
- Established optimal conditions for conditioning, retention, derivatization, and elution.
- Developed calibration graphs for various primary amines (methylamine to beta-phenylethylamine) with detection limits between 0.01-0.06 mg L(-1).
- Demonstrated the method's applicability for determining total primary aliphatic amine concentration and primary amino groups in real water samples.
Conclusions:
- The developed SPE-OPA-NAC method offers a sensitive and reliable approach for primary amine determination in water.
- This technique enhances analytical capabilities for environmental monitoring and water quality assessment.
- The method allows for the estimation of total primary aliphatic amine concentration and -NH(2) group levels.