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Published on: March 17, 2013
CD5+ B cells predominate in peripheral tissues of rabbit
1Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153.
Journal of Immunology (Baltimore, Md. : 1950)
|December 15, 1992
Summary
Nearly all adult rabbit B lymphocytes express CD5, a stark contrast to mice and humans. This suggests early development and self-renewal maintain the rabbit B cell population throughout life.
Area of Science:
- Immunology
- Cell Biology
Background:
- Restricted usage of VH genes is noted in rabbit B lymphocytes and human/murine CD5 B lymphocytes.
- This observation suggests a potential high prevalence of CD5+ B lymphocytes in rabbits.
Purpose of the Study:
- To investigate the expression of CD5 on rabbit B lymphocytes.
- To determine the frequency of CD5+ B lymphocytes in peripheral lymphoid tissues of adult rabbits.
Main Methods:
- Cloned the rabbit CD5 gene using human CD5 cDNA probe.
- Developed anti-rabbit CD5 monoclonal antibodies (mAb) via transfection and immunization.
- Analyzed CD5 expression on rabbit lymphocytes using two-color immunofluorescence and Western blot.
Main Results:
- Successfully cloned the rabbit CD5 gene and developed specific mAb.
- Western blot confirmed a 67-kDa CD5 protein in rabbit lymphoid tissues.
- Two-color immunofluorescence revealed that essentially all peripheral B lymphocytes in adult rabbits express CD5.
Conclusions:
- Rabbit peripheral B lymphocytes predominantly express CD5, unlike in mice and humans.
- This unique expression pattern may indicate early ontogeny and self-renewal as the primary maintenance mechanism for rabbit B cells.
