Detecting haplotypes of DXS7132 and DXS6804 loci by multiplex PCR

DXS7132 and DXS6804 were amplified by means of multiplex PCR and detected by polyacrylamide gel electrophoresis and silver stain. Population study has been carried out in Guangdong Han Population of South China. DXS7132 exhibited 7 distinguishable alleles ranging from 276-300 bp in size. Number of alleles at DXS6804 was 6 and its range was 177-201 bp. Sequencing revealed that their common sequence motifs were tetranucleotide repeats. On the genomic mapping information linkage disequilibrium between DXS7132 and DXS6804 was shown. Female haplotypes were determined by analyzing family trios. In 827(211 males, 616 females) chromosomes, total 33 haplotypes were identified. Deviation from Hardy-Weinberg equilibrium was not detected in the female diploid data. Gene diversity, polymorphism information content and probability of exclusion for the two loci were 0.9999, 0.9440 and 0.9411, respectively. Investigation in 308 family trios suggested a co-dominant X-linked inheritance and mutations were not found. The result indicates that DXS7132 and DXS7132 will be very useful for paternity testing that involve a female child, especially for the deficiency parents cases.