Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved protocol for SAGE tag-to-gene allocation.

Ute Kannbley1, Krisztian Kapinya, Ulrich Dirnagl

  • 1Humboldt University Berlin, Germany

Biotechniques
|June 20, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Striking a balance: A proposed benefit assessment matrix for ethical animal research.

Neuroscience applied·2026
Same author

Stroke Action Plan for Europe 2018-2030 (SAP-E): mid-term review and update.

European stroke journal·2026
Same author

How to integrate patient and carer perspectives, methodological rigor, and ethics into biomedical research funding.

PLoS biology·2025
Same author

Whole brain volume loss is associated with a short-term disability progression in relapse-activity free multiple sclerosis.

Journal of neurology·2025
Same author

Clinical translation of 3D-printed patient-specific bone implants: a consensus statement.

International journal of surgery (London, England)·2025
Same author

Randomized Controlled Preclinical Trial of a Benzodiazepine-Dihydropyridine Hybrid Molecule in Rodent Stroke.

Journal of the American Heart Association·2025
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

This study introduces an improved Serial Analysis of Gene Expression (SAGE) protocol for accurate gene identification. The enhanced method improves tag-to-gene allocation, aiding gene discovery and expression analysis.

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Serial Analysis of Gene Expression (SAGE) is a powerful tool for large-scale gene expression analysis and discovery.
  • Accurate tag-to-gene allocation in SAGE can be challenging due to ambiguous sequence tags or novel genes.

Purpose of the Study:

  • To develop an improved protocol for precise identification of genes corresponding to SAGE tags.
  • To enhance the reliability of SAGE for gene discovery and quantitative expression analysis.

Main Methods:

  • Developed a protocol involving isolation of 3'-terminal cDNA restriction fragments using paramagnetic streptavidin beads.
  • Ligated linkers before amplification and utilized linker- and tag-specific primers for stringent PCR.
  • Leveraged the 3'-terminal location of SAGE tags relative to the NlaIII restriction site.

Related Experiment Videos

Main Results:

  • Successfully improved the accuracy of tag-to-gene allocation in SAGE experiments.
  • The protocol allows for stringent PCR conditions, enhancing specificity.
  • Demonstrated that the method provides quantitative information for further expression analysis.

Conclusions:

  • The improved SAGE protocol effectively addresses limitations in tag-to-gene identification.
  • This method enhances the utility of SAGE for both gene discovery and quantitative expression profiling.
  • The protocol offers a more robust approach for analyzing gene expression patterns.