Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray-based method for combinatorial library sequence mapping and characterization.

V Abécassis1, L Jaffrelo, D Rickman

  • 1Centre National de la Recherche Scientifique, UPR 2167, Gifsur-Yvette, France.

Biotechniques
|June 20, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Instantons and the Path to Intermittency in Turbulent Flows.

Physical review letters·2022
Same author

Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Society, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, London, Ont. Sept. 15-18, 2011.

Canadian journal of surgery. Journal canadien de chirurgie·2022
Same author

Treating Brain Metastases from Breast Cancer: Outcomes after Stereotactic Radiosurgery.

Clinical oncology (Royal College of Radiologists (Great Britain))·2020
Same author

Prognostic impact of baseline tumour immune infiltrate on disease-free survival in patients with completely resected, BRAF<sup>v600</sup> mutation-positive melanoma receiving adjuvant vemurafenib.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

Pharmacodynamics and pharmacokinetics of insulin detemir and insulin glargine 300 U/mL in healthy dogs.

Domestic animal endocrinology·2018
Same author

Correction to: Variations of characteristic time scales in rotating stratified turbulence using a large parametric numerical study.

The European physical journal. E, Soft matter·2017
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

This study introduces a DNA-chip method for rapid DNA sequence mapping using competitive hybridization. This technique enhances accuracy for analyzing gene family DNA shuffling and directed evolution libraries.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • High-throughput DNA sequence mapping is crucial for genetic analysis and biotechnology.
  • Current methods can be sensitive to experimental variations, limiting accuracy.
  • Analyzing combinatorial libraries, especially from DNA shuffling, presents unique mapping challenges.

Purpose of the Study:

  • To develop and present a novel DNA-chip-based method for high-throughput DNA sequence mapping.
  • To improve sequence discrimination and reduce sensitivity to experimental conditions in hybridization assays.
  • To apply this method for characterizing recombination biases in directed evolution libraries.

Main Methods:

  • Utilized a DNA-chip platform for competitive hybridization assays.

Related Experiment Videos

  • Employed short, differentially labeled fluorescent oligonucleotide probes against glass-supported PCR products.
  • Optimized hybridization by using an excess of probes targeting the same sequence segment.

    • Main Results:

    • The competitive hybridization approach significantly improved sequence discrimination.
    • The method demonstrated reduced sensitivity to variations in probe concentration, temperature, and duration.
    • Successfully applied the technique to map and characterize recombination biases in DNA shuffling-derived combinatorial libraries.

    Conclusions:

    • The developed DNA-chip method offers a robust and accurate solution for high-throughput sequence mapping.
    • This technique is particularly well-suited for analyzing complex combinatorial libraries in genetic engineering.
    • The findings provide insights into recombination biases, aiding directed evolution strategies.