Jove
Visualize
Contact Us

Related Experiment Videos

Decoding the LIM development code.

Gordon N Gill

    Transactions of the American Clinical and Climatological Association
    |June 20, 2003
    PubMed
    Summary
    This summary is machine-generated.

    Cell fate determination relies on LIM homeodomain protein combinations, forming molecular codes. These codes, like homotetramers and hexamers involving nuclear LIM interactor (NLI), dictate specific cell types, such as motor neurons.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Viewing serine/threonine protein phosphatases through the eyes of drug designers.

    The FEBS journal·2013
    Same author

    Bio-molecular architects: a scaffold provided by the C-terminal domain of eukaryotic RNA polymerase II.

    Nano reviews·2011
    Same author

    Structural and functional analysis of the phosphoryl transfer reaction mediated by the human small C-terminal domain phosphatase, Scp1.

    Protein science : a publication of the Protein Society·2010
    Same author

    Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity.

    Development (Cambridge, England)·2009
    Same author

    Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1.

    Molecular cell·2006
    Same author

    The Grainyhead-like epithelial transactivator Get-1/Grhl3 regulates epidermal terminal differentiation and interacts functionally with LMO4.

    Developmental biology·2006
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Developmental Biology
    • Molecular Biology
    • Genetics

    Background:

    • Cell differentiation arises from progenitor cells.
    • Ligands and cell surface receptors regulate gene expression via transcriptional regulators.
    • LIM homeodomain proteins are key regulators of cell fate determination.

    Purpose of the Study:

    • Investigate the molecular basis of the LIM domain "code" in cell fate determination.
    • Understand how combinations of LIM homeodomain proteins specify cell fates.
    • Explore the role of nuclear LIM interactor (NLI) in forming these codes.

    Main Methods:

    • Analysis of protein complex formation.
    • Studying gene expression patterns in model organisms (Drosophila, chick).
    • Investigating the function of LIM homeodomain proteins and NLI.

    Related Experiment Videos

    Main Results:

    • A basic code involves a homotetramer of 2 LIM homeodomain proteins bridged by NLI.
    • A complex code uses a hexamer complex of NLI, Lhx3, and Isl1 to determine ventral motor neuron formation.
    • The same molecular "words" can have different meanings based on expression context.

    Conclusions:

    • LIM homeodomain protein combinations form a molecular code for cell fate determination.
    • The context-dependent nature of this code allows for diverse cell fates.
    • This provides a framework for understanding complex developmental processes.