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Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple
Suzanne D Vernon1, Daniel H Farkas, Elizabeth R Unger
1Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. svernon@cdc.gov
Background:
We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor) in identifying multiple pathogens.
Methods:
Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.
Results:
Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.
Conclusions:
This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.