Related Experiment Videos
[Change in spectral properties of nitrosohemoglobin induced by UV light]
O V Putintseva1, V G Artiukhov, E A Kalaeva
1Voronezh State University, Universitetskaya pl. 1, Voronezh, 394693 Russia.
Biofizika
|June 21, 2003
Abstract:
The effect of UV radiation on the spectral characteristics of human nitrosohemoglobin in a wide dose range (151-4530 J/m2) was studied. It was shown that the irradiation of hemoprotein solutions with low doses of UV light (151-453 J/m2) led to local conformational rearrangements of the iron porphyrin moiety of the molecule. The apoprotein exhibited a high photostability under the conditions of the experiment. High doses of UV radiation (1359-4530 J/m2) induced the accumulation of methemoglobin in the protein sample. A scheme of the photoinduced formation of methemoglobin was elaborated.