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Related Experiment Videos

Proton transport by gastric membrane vesicles.

H Chang, G Saccomani, E Rabon

    Biochimica Et Biophysica Acta
    |January 21, 1977
    PubMed
    Summary

    Hog gastric membrane fractions show ATP-dependent proton (H+) uptake, suggesting a role for a dimeric (H+ + K+)-ATPase in gastric acid secretion. This enzyme is likely non-electrogenic.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Physiology

    Background:

    • Gastric acid secretion is crucial for digestion and involves proton pumps in the gastric mucosa.
    • Understanding the mechanisms of proton transport is essential for comprehending gastric physiology and related disorders.

    Purpose of the Study:

    • To isolate and characterize the enzyme responsible for proton (H+) uptake in hog gastric mucosa.
    • To elucidate the properties and function of the identified ATPase in proton transport.

    Main Methods:

    • Differential and density gradient centrifugation, and free flow electrophoresis were used to purify membrane fractions from hog gastric mucosa.
    • Cation-activated ouabain-insensitive ATPase activity and ATP-dependent H+ uptake were measured.
    • Antibody binding studies and the effects of ionophores and inhibitors were analyzed.

    Main Results:

    • A membrane fraction 35-fold enriched in cation-activated ouabain-insensitive ATPase was isolated.
    • This fraction exhibited ATP-dependent H+ uptake into an osmotically sensitive space.
    • The H+ uptake demonstrated specificity for ATP, a requirement for cations (K+ > Rb+ > Cs+ > Na+ > Li+), and was inhibited by DCCD.
    • Maximal H+ uptake occurred with an outward K+ gradient, and ionophores like valinomycin and nigericin modulated the H+ gradient, indicating vesicle properties.

    Conclusions:

    • The results strongly suggest that H+ uptake is mediated by a dimeric (H+ + K+)-ATPase.
    • The enzyme is likely located on the luminal surface of gastric glands.
    • The proton transport mechanism is probably non-electrogenic.

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