Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kininogen deficiency in the rat.

S Oh-ishi1, I Hayashi, K Yamaki

  • 1Department of Pharmacology, School of Pharmac. Sci., Kitasato Univ., Tokyo, Japan.

Agents and Actions. Supplements
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A clustering approach to identify and characterize the asthma and chronic obstructive pulmonary disease overlap phenotype.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2017
Same author

Mucous membrane pemphigoid with generalized blisters: IgA and IgG autoantibodies target both laminin-332 and type XVII collagen.

The British journal of dermatology·2011
Same author

Neutralization of the γ-secretase activity by monoclonal antibody against extracellular domain of nicastrin.

Oncogene·2011
Same author

Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis.

International journal of oncology·2011
Same author

Hepatitis B virus DNA integration frequently observed in the hepatocellular carcinoma DNA of hepatitis C virus-infected patients.

International journal of oncology·2011
Same author

Deletion type hepatocyte growth factor has different effects on growth and c-met expression in 10 different human hepatocellular carcinoma cell lines.

International journal of oncology·2011

Brown Norway Katholiek (B/N-Ka) rats have deficient plasma kininogens due to a secretory defect, not synthesis issues. This unique rat strain aids in differentiating inflammatory responses involving the kinin system.

Area of Science:

  • Biochemistry
  • Immunology
  • Physiology

Background:

  • The Brown Norway Katholiek (B/N-Ka) rat strain is uniquely deficient in plasma high-molecular-weight (HMW) and low-molecular-weight (LMW) kininogens.
  • This deficiency affects prekallikrein levels, impacting the kinin system.

Purpose of the Study:

  • To develop and utilize radioimmunoassays (RIAs) for quantifying rat kininogens.
  • To investigate the cause of plasma kininogen deficiency in B/N-Ka rats.
  • To assess the role of kinins in different inflammatory models using the B/N-Ka strain.

Main Methods:

  • Development of RIAs for rat HMW-kininogen, LMW-kininogen, and T-kininogen.
  • Measurement of plasma kininogen levels in B/N-Ka and normal B/N-Ki rats.
  • Analysis of kininogen synthesis and secretion using 35S-methionine uptake in primary hepatocyte cultures.

Related Experiment Videos

  • Northern blot analysis of liver mRNA to assess kininogen gene expression.

    • Main Results:

    • B/N-Ka rats exhibited approximately 3% of normal plasma levels for HMW- and LMW-kininogens.
    • Kininogens were synthesized in B/N-Ka rat livers but not secreted into the medium, indicating a secretory defect.
    • Northern blot confirmed the presence and normal size/intensity of HMW-kininogen mRNA in B/N-Ka rat livers.
    • B/N-Ka rats showed reduced inflammatory responses to carrageenin and kaolin but normal responses to phorbol ester (PMA) and zymosan.

    Conclusions:

    • The plasma deficiency of kininogens in B/N-Ka rats is due to a post-synthesis secretory defect.
    • Kinin plays a significant role in exudation in carrageenin- and kaolin-induced edema.
    • The B/N-Ka rat strain is a valuable tool for distinguishing inflammatory models that involve the kinin system.