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Updated: Jul 21, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
Published on: March 3, 2015
Organization of cell-regulatory systems through modular-protein-interaction domains
1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada. pawson@mshri.on.ca
Abstract:
Cells are organized through the interactions of proteins with one another, and with phospholipids, nucleic acids and small molecules, suggesting a molecular recognition code for cell assembly. These interactions are mediated by a set of conserved protein interaction domains, which regulate the dynamic behaviour of the cell, and are used to build protein complexes and networks with emergent properties. Cell regulatory proteins have a modular architecture, which may have facilitated the evolution of new signalling pathways, and is exploited by pathogenic micro-organisms and chimeric oncoproteins to re-specify cellular function.
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