Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A CD40 bridge between innate and adaptive immunity.

Edward A Clark1, Andrew Craxton

  • 1Department of Microbiology, Box 357242, University of Washington, Seattle, WA 98195, USA.

Immunity
|June 24, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Species-specific cleavage of the autophagy adaptor p62 dictates responses to TNF.

Molecular cell·2026
Same author

PAXX/Ku interaction is rate limiting for repair of double-strand DNA breaks requiring end processing.

The Journal of biological chemistry·2025
Same author

TAp73 regulates mitochondrial dynamics and multiciliated cell homeostasis through an OPA1 axis.

Cell death & disease·2024
Same author

A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.

Immunity·2024
Same author

Cleavage of cFLIP restrains cell death during viral infection and tissue injury and favors tissue repair.

Science advances·2023
Same author

Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade.

Nature communications·2023
Same journal

Targeting cholesterol esterification sensitizes liver cancer to CD8<sup>+</sup> T cell attack by impairing metabolic and redox resilience.

Immunity·2026
Same journal

Brain endothelial cells orchestrate a neuroprotective antiviral state in the CNS in response to peripheral viral pattern sensing.

Immunity·2026
Same journal

Extracellular ATP-P2RY2 signaling drives intratumoral prostaglandin E2 accumulation and adaptive resistance to immunotherapy in solid tumors.

Immunity·2026
Same journal

B cell-derived type I interferon sustains T cell functionality upon strong TCR stimulation during chronic infection.

Immunity·2026
Same journal

Lactate binds and inhibits the innate immune sensor STING to promote tumor immune evasion.

Immunity·2026
Same journal

Antibody binding geometry and affinity control inhibitory hFcγRIIB receptor signaling.

Immunity·2026
See all related articles

C4b binding protein (C4BP) binds to CD40 receptors on B cells, activating them. This discovery reveals a new mechanism for CD40 in linking innate and adaptive immunity.

Area of Science:

  • Immunology
  • Complement System
  • B cell Biology

Background:

  • The classical complement (C) pathway is crucial for innate immunity.
  • Complement component 4b-binding protein (C4BP) is a known regulator of the classical C pathway.
  • CD40 receptors on B cells play a vital role in adaptive immune responses.

Purpose of the Study:

  • To investigate the interaction between C4BP and B cells.
  • To determine if C4BP can modulate B cell function via CD40.
  • To explore a novel role for CD40 in bridging innate and adaptive immunity.

Main Methods:

  • Co-immunoprecipitation assays to detect C4BP binding to CD40.
  • Flow cytometry to assess B cell activation markers.
  • Functional assays to measure B cell proliferation and antibody production.

Related Experiment Videos

Main Results:

  • C4BP directly binds to CD40 receptors on B cells.
  • This binding event leads to the activation of B cells.
  • C4BP-mediated CD40 activation enhances B cell responses.

Conclusions:

  • C4BP acts as a ligand for CD40 on B cells.
  • CD40 signaling can be initiated by complement proteins.
  • This interaction provides a novel link between complement-mediated innate immunity and adaptive B cell responses.