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Updated: Sep 25, 2026

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Characterization of a Tal/SCL-like transcription factor in the pacific oyster Crassostrea gigas
Catherine Barreau-Roumiguière1, Caroline Montagnani, Jean Michel Escoubas
1Laboratoire de Défense et Résistance chez les Invertébrés Marins (DRIM), UMR5098 (IFREMER, CNRS, UMII) Université de Montpellier II, place Eugène Bataillon, CC80, 34095, Montpellier, France.
Abstract:
We have cloned and characterized a cDNA encoding Cg-tal in the Pacific oyster Crassostrea gigas. The isolated cDNA encodes a 219 amino acids protein that contains the basic helix-loop-helix (bHLH) domain homologous to that of vertebrate and invertebrate Tal1/SCL. Phylogenetic analyses sustained that Cg-Tal belongs to this family of bHLH transcription factors. Northern blot analysis of Cg-tal mRNA expression in adult oyster tissues indicated that Cg-ta1 was specifically expressed in hemocytes, in a constitutive manner. In vertebrates, activation of Tal1/SCL expression is essential for the initiation of hematopoiesis and the formation of hematopoietic stem cells. Considering Tal1/SCL function in vertebrates, Cg-Tal is likely to constitute a promising tool for studying hematopoiesis in oyster.
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