Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences in Ca(2+)-mediation of hypotonic and Na(+)-nutrient regulatory volume decrease in suspensions of jejunal

R J MacLeod1, P Lembessis, J R Hamilton

  • 1Department of Paediatrics, McGill University-Montreal Children's Hospital Research Institute, Quebec, Canada.

The Journal of Membrane Biology
|October 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scanning laser-induced endothelial injury: a standardized and reproducible thrombosis model for intravital microscopy.

Scientific reports·2022
Same author

Investigation of anemia of unknown origin.

Immunohematology·2021
Same author

Inhibition of protease-activated receptor 4 impairs platelet procoagulant activity during thrombus formation in human blood.

Journal of thrombosis and haemostasis : JTH·2016
Same author

Fatal hemorrhage from simple lacerations of the scalp.

Forensic science, medicine, and pathology·2015
Same author

Safety and efficacy of targeting platelet proteinase-activated receptors in combination with existing anti-platelet drugs as antithrombotics in mice.

British journal of pharmacology·2012
Same author

Transgenic, inducible RNAi in megakaryocytes and platelets in mice.

Journal of thrombosis and haemostasis : JTH·2010

Regulatory Volume Decrease (RVD) in jejunal enterocytes differs based on the swelling trigger. Hypotonic RVD requires extracellular calcium and calmodulin, while nutrient-induced RVD involves different signaling pathways.

Area of Science:

  • Physiology
  • Cell Biology
  • Gastroenterology

Background:

  • Jejunal villus enterocytes regulate cell volume through Regulatory Volume Decrease (RVD).
  • RVD is crucial for maintaining intestinal homeostasis and function.
  • Understanding the ion transport mechanisms underlying RVD is essential.

Purpose of the Study:

  • To investigate the distinct calcium (Ca2+) signaling pathways and ion conductances involved in RVD of jejunal villus enterocytes.
  • To differentiate RVD mechanisms triggered by passive hypotonic swelling versus nutrient absorption (D-glucose or L-alanine).

Main Methods:

  • Cell volume was measured using electronic cell sizing.
  • Experiments were conducted in nominally Ca2+-free media and with calcium chelators (EGTA, BAPTA-AM).

Related Experiment Videos

  • Ion channel blockers (niguldipine, nifedipine, diltiazem, Ni2+, Co2+, charybdotoxin) and calmodulin antagonists (W-7, W-13) were used to probe signaling pathways.

    • Main Results:

    • Hypotonic RVD necessitates extracellular Ca2+ and is inhibited by Ca2+ channel blockers and calmodulin antagonists W-7 and W-13.
    • L-alanine-induced RVD is not significantly affected by Ca2+-free conditions or Ca2+ channel blockers but is influenced by nutrient absorption.
    • D-glucose-induced RVD is inhibited by charybdotoxin, suggesting a role for Ca2+-activated K+ channels, while hypotonic RVD involves a calmodulin-dependent, charybdotoxin-insensitive K+ conductance.

    Conclusions:

    • Hypotonic RVD in jejunal enterocytes relies on extracellular Ca2+ and a calmodulin-activated, charybdotoxin-insensitive K+ conductance.
    • Nutrient-induced RVD (D-glucose and L-alanine) employs distinct signaling mechanisms, highlighting the complexity of volume regulation in enterocytes.