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Related Experiment Videos

Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays.

Hidemasa Bono1, Ken Yagi, Takeya Kasukawa

  • 1Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

Genome Research
|June 24, 2003
PubMed
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This summary is machine-generated.

Researchers mapped mouse mRNA expression across 20 tissues, identifying 7,206 tissue-specific clones and revealing novel non-coding RNAs. This functional genomics data offers insights into gene function and tissue biology.

Area of Science:

  • Genomics
  • Transcriptomics
  • Functional Genomics

Background:

  • The RIKEN Mouse Gene Encyclopedia project significantly expanded known mouse mRNA transcripts.
  • Validating reproducible gene expression in tissues is crucial for functional genomics studies.

Purpose of the Study:

  • To determine the expression profile of 57,931 clones across 20 mouse tissues.
  • To identify tissue-specific gene expression and analyze their functions.
  • To investigate the expression of non-protein-coding mRNAs.

Main Methods:

  • Utilized cDNA microarrays to analyze gene expression profiles of 57,931 clones in 20 mouse tissues.
  • Applied stringent criteria to identify tissue-specific clones.
  • Assigned Gene Ontology terms to characterized clones.

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Main Results:

  • Identified 7,206 clones with stringent tissue-specific expression.
  • Characterized the molecular function ontology for tissue-specific clones.
  • Confirmed reproducible expression for numerous unknown and unclassifiable ESTs and identified novel non-coding RNAs.

Conclusions:

  • The study provides valuable insights into mouse tissue function through comprehensive gene expression profiling.
  • The data supports the functional genomics of identified transcriptional units and non-coding RNAs.
  • This expression atlas serves as a resource for understanding mouse biology and gene function.