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Updated: Sep 12, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
Electrospray ionization mass spectrometry of AZT H-phosphonates conjugated with steroids
Qiang Xiao1, Yong Ju, Xinping Yang
1The Key Laboratory of Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China.
Abstract:
AZT H-phosphonates conjugated with steroids were synthesized and determined by positive and negative ion electrospray ionization mass spectrometry (ESI-MS) in conjunction with tandem mass spectrometry (MS/MS). The fragmentation pathways were investigated in detail. There are very different characteristic fragment ions in the positive and negative ion MS/MS spectra. The azide group of compounds 6a and 6b underwent either elimination of HN(3) or rearrangement to an amine in both positive and negative ion mass spectrometry.
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