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An efficient chemical method for dephosphorylation of phosphopeptides
Hiroki Kuyama1, Chikako Toda, Makoto Watanabe
1Life Science Laboratory, Shimadzu Corporation, Kyoto 604-8511, Japan.
Rapid Communications in Mass Spectrometry : RCM
|June 24, 2003
Abstract:
Phosphate moieties found on serine, threonine or tyrosine residues in peptides, e.g., in proteolytic digests of proteins, were cleaved using hydrofluoric acid or hydrogen fluoride-pyridine without side reactions.