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Local administration of methotrexate bound to activated carbon particles (MTX-CH) for treating cancers in mice
Tadao Ito1, Akeo Hagiwara, Tsuyoshi Takagi
1Department of Digestive Surgery, Kyoto Prefectural University of Medicine, 465 Kajiicho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, Japan 602-8566. titoh@frontier.kyoto-u.ac.jp
Purpose:
A new dosage formulation of methotrexate (MTX-CH) was developed to control cancer growth by local administration.
Materials And Methods:
BALB/c mice received a subcutaneous inoculation with transplantable Colon 26 cancer cells on the back. When cancerous tumor became 7 mm in diameter, MTX-CH or MTX aqueous solution (MTX-sol) was injected into the tumor. The MTX concentration in the tumor was compared between the MTX-CH group and the MTX-sol group. The tumor growth was assessed in single or repeated local administration experiments.
Results:
The MTX concentrations were significantly higher for longer periods in the MTX-CH group than those in the MTX-sol group. Repeated MTX-CH administration was significantly more effective for suppressing the tumor growth compared with repeated MTX-sol administration.
Conclusion:
MTX-CH is superior to MTX-sol in controlling the tumor growth by local administration because of its long-acting effect.
