Related Experiment Video
Updated: Aug 3, 2026
Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
Published on: September 25, 2012
Expression of p33ING1 mRNA and chemosensitivity in brain tumor cells
G Tallen1, K Riabowol, J E Wolff
1Kinderklinik m. S. Hämatologie/Onkologie, Charité, Campus Virchow Klinikum, Med. Fakultät der Humboldt-Universität Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. gesche.tallen@charite.de
Background:
Mutations and down-regulation of tumor suppressor genes can contribute to both tumorigenesis and chemotherapy resistance. The tumor suppressor p33ING1 has growth-inhibitory and pro-apoptotic effects recruiting p53 and it plays a role in DNA repair through interaction with PCNA. We questioned whether p33ING1 mRNA expression correlates with the chemosensitivity of brain tumor cells.
Materials And Methods:
Various malignant brain tumor cell lines were examined for their sensitivity to cisplatin, doxorubicin, etoposide and the antimitotic agents vincristine and paclitaxel by MTT-cytotoxicity assays. p33ING1 mRNA expression was determined by RT-PCR.
Results:
We found that, unlike other tumor types, ING1 levels were higher in glioma cell lines than in normal control cells. Medulloblastoma cells revealed the lowest ING1 expression of the lines tested. Comparing all cell lines, p33ING1 gene expression significantly (p = 0.028) correlated with resistance to vincristine (r2 = 0.87).
Conclusion:
Our results suggest that p33ING1 mRNA levels may be used to predict the chemosensitivity of brain tumor cells to vincristine.
Insights
p33ING1 gene expression in brain tumors correlates with resistance to vincristine chemotherapy. Higher p33ING1 mRNA levels may predict reduced sensitivity to this specific anti-cancer drug.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Tumor suppressor genes, like p33ING1, are crucial in controlling cell growth and apoptosis.
- p33ING1 plays a role in DNA repair and interacts with proteins such as p53 and PCNA.
- Dysregulation of tumor suppressors can lead to cancer development and resistance to chemotherapy.
Purpose of the Study:
- To investigate the correlation between p33ING1 mRNA expression and the chemosensitivity of malignant brain tumor cells.
- To determine if p33ING1 levels can predict treatment response in brain tumors.
Main Methods:
- Utilized MTT-cytotoxicity assays to assess the sensitivity of various brain tumor cell lines to chemotherapeutic agents (cisplatin, doxorubicin, etoposide, vincristine, paclitaxel).
- Quantified p33ING1 mRNA expression using reverse transcription-polymerase chain reaction (RT-PCR).
Main Results:
- Glioma cell lines exhibited higher ING1 mRNA levels compared to normal control cells.
- Medulloblastoma cell lines showed the lowest ING1 expression among the tested lines.
- A significant positive correlation (p = 0.028, r² = 0.87) was observed between p33ING1 gene expression and resistance to vincristine across all cell lines.
Conclusions:
- p33ING1 mRNA levels can potentially serve as a predictive biomarker for brain tumor cell sensitivity to vincristine.
- This finding may aid in personalizing chemotherapy strategies for brain tumor patients.
More Related Videos
06:32Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
09:40Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
