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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Published on: October 28, 2009
Granulocyte-colony stimulating factor stimulates immunoreactive endothelin-1 release from cultured bovine endothelial
A Namiki1, Y Hirata, M Fukazawa
1Third Department of Internal Medicine, Toho University School of Medicine, Tokyo, Japan.
Abstract:
We examined the effect of human recombinant granulocyte-colony stimulating factor (G-CSF) on the release of immunoreactive endothelin-1 (ET-1) from cultured bovine vascular endothelial cells. G-CSF dose dependently (10(-8)-10(-6) M) increased the release of immunoreactive ET-1 as a function of time under a serum-free condition. Coaddition of G-CSF and thrombin induced an additive effect on immunoreactive ET-1 release. Neither Ca2+ channel antagonist nor cyclooxygenase inhibitor affected immunoreactive ET-1 release stimulated by G-CSF. These results suggest that G-CSF, in addition to its effect on granulocyte progenitors, has a direct effect on vascular endothelium to induce the release of immunoreactive ET-1.
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