Related Experiment Videos
A comparison of procedures for analysing microsatellite (CA)-repeat polymorphisms
B B Cohen1, M R Wallace, D N Crichton
1MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Molecular and Cellular Probes
|October 1, 1992
Abstract:
Dinucleotide repeat sequences ('microsatellites') have been used as polymorphic genetic markers following amplification in the polymerase chain reaction (PCR). We have compared several methods of analysing the PCR products. The most reliable and unambiguous results were obtained when the PCR products were probed with a specific dinucleotide repeat oligonucleotide, so that only the microsatellite-containing products were detectable.