Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A comparison of procedures for analysing microsatellite (CA)-repeat polymorphisms.

B B Cohen1, M R Wallace, D N Crichton

  • 1MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.

Molecular and Cellular Probes
|October 1, 1992
PubMed
Summary

Analyzing polymerase chain reaction (PCR) products for dinucleotide repeat sequences, also known as microsatellites, requires reliable methods. Probing PCR products with specific oligonucleotides offers the most accurate detection of microsatellite markers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations of <i>P2RX7</i> Functional Diplotypes with Localized Aggressive Periodontitis.

JDR clinical and translational research·2019
Same author

Genetic polymorphisms and periodontal disease in populations of African descent: A review.

Journal of periodontal research·2017
Same author

Versatile and precise gene-targeting strategies for functional studies in mammalian cell lines.

Methods (San Diego, Calif.)·2017
Same author

The primacy of NF1 loss as the driver of tumorigenesis in neurofibromatosis type 1-associated plexiform neurofibromas.

Oncogene·2017
Same author

Serologically detectable H-Y ('male') antigen: Mr or myth?

Immunology today·2014
Same author

H-Y antigen and sex determination.

Immunology today·2014

Area of Science:

  • Genetics
  • Molecular Biology

Background:

  • Dinucleotide repeat sequences, or microsatellites, are valuable polymorphic genetic markers.
  • Polymerase chain reaction (PCR) is commonly used to amplify these sequences for analysis.

Purpose of the Study:

  • To compare different methods for analyzing PCR-amplified microsatellite sequences.
  • To identify the most reliable and unambiguous method for microsatellite detection.

Main Methods:

  • Comparison of various analytical techniques for PCR products.
  • Application of specific dinucleotide repeat oligonucleotide probes for detection.

Main Results:

  • Probing PCR products with specific dinucleotide repeat oligonucleotides yielded the most reliable results.

Related Experiment Videos

  • This method allowed for unambiguous detection of microsatellite-containing PCR products.

    • Conclusions:

    • Specific oligonucleotide probing is a superior method for analyzing microsatellite markers.
    • This technique enhances the accuracy and clarity of genetic marker analysis in PCR studies.