Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of a New Submaximal Walk Test to Predict Maximal Oxygen Consumption in Healthy Adults.

Sensors (Basel, Switzerland)·2021

Same author

A clinical study of submandibular schwannoma.

Oral surgery, oral medicine, oral pathology and oral radiology·2021

Same author

Effective Cryopreservation of a Bioluminescent Auxotrophic <i>Escherichia coli</i>-Based Amino Acid Array to Enable Long-Term Ready-to-Use Applications.

Biosensors·2021

Same author

Factors for return to emergency department and hospitalization in elderly urinary tract infection patients.

The American journal of emergency medicine·2021

Same author

2020 Korean Consensus Guidelines for Diagnosis and Treatment of Chronic Hand Eczema.

Annals of dermatology·2021

Same author

Age-related differences in revisits to the emergency departments of eight Korean university hospitals.

Archives of gerontology and geriatrics·2021

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

An azophenol-based chromogenic pyrophosphate sensor in water.

Dong Hoon Lee¹, Ja Hyun Im, Seung Uk Son

¹Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.

Journal of the American Chemical Society

|June 26, 2003

Summary

A novel azophenol sensor selectively detects pyrophosphate in water. This colorimetric method offers a simple way to identify pyrophosphate ions in solutions.

More Related Videos

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jul 15, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

Area of Science:

Analytical Chemistry
Materials Science

Background:

Pyrophosphate is an important analyte in biological and environmental systems.
Selective and sensitive detection methods for pyrophosphate are crucial.

Purpose of the Study:

To develop a new colorimetric sensor for pyrophosphate detection.
To investigate the selectivity and sensitivity of the sensor in aqueous solutions.

Main Methods:

Synthesis of an azophenol-based colorimetric probe.
Spectrophotometric analysis of the sensor's response to pyrophosphate.
Evaluation of sensor selectivity against other anions.

Main Results:

The azophenol sensor exhibited a distinct color change upon binding with pyrophosphate.
High selectivity for pyrophosphate was observed over other common anions.
The sensor demonstrated good sensitivity in aqueous media.

Conclusions:

A new azophenol-based colorimetric sensor provides a selective method for pyrophosphate detection.
This sensor holds potential for applications in environmental monitoring and biochemical analysis.