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Measurement of HIV-1 reverse transcriptase by a nonradioactive assay system
F Hammerschmid1, E Maier, B Rosenwirth
1Sandoz Forschungsinstitut Wien, Austria.
Annals of the New York Academy of Sciences
|November 30, 1992
Abstract:
Reverse transcriptase activity was measured by incorporation of dUMP linked to digoxigenin into a suitable template-primer molecule. Incorporation was monitored by using peroxidase-conjugated Fab fragments directed against digoxigenin. The standard assay measuring incorporation of radiolabeled nucleotides into acid-precipitable material was compared with this new immunochemical assay with regard to its usefulness for testing inhibitors of reverse transcriptase.