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Twelve-week RBC storage.

John R Hess1, Heather R Hill, Cindy K Oliver

  • 1Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC, USA. jhess@umm.edu

Transfusion
|June 26, 2003
PubMed
Summary
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Storing red blood cells (RBCs) in lower salt solutions like EAS-76v6 extends storage life to 12 weeks. This method maintains acceptable recovery and minimizes hemolysis, improving RBC availability and safety.

Area of Science:

  • Biomedical science
  • Blood banking
  • Red blood cell preservation

Background:

  • Improving red blood cell (RBC) availability and safety is crucial.
  • Optimizing RBC adenosine triphosphate (ATP) production and minimizing hemolysis are key to extending storage duration.

Purpose of the Study:

  • To evaluate the efficacy of experimental additive solution 76 (EAS-76) variants with varying NaCl concentrations for extended RBC storage.
  • To compare the in vivo recovery of RBCs stored in AS-1 versus a novel EAS-76 variant (EAS-76v6).

Main Methods:

  • Packed RBCs were stored in four EAS-76 variants (30-45 mEq/L NaCl) and assessed weekly for 12 weeks.
  • A crossover study compared 6-week storage in AS-1 with 12-week storage in EAS-76v6 (30 mEq/L NaCl) using in vivo recovery in volunteers.

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Main Results:

  • Lower NaCl concentrations in EAS-76 correlated with higher RBC ATP, reduced hemolysis, and less microvesiculation.
  • RBC 2,3-diphosphoglycerate (2,3-DPG) levels were preserved for two weeks.
  • RBCs stored for 12 weeks in EAS-76v6 showed a 78% +/- 4% 24-hour in vivo recovery.

Conclusions:

  • Extended storage of RBCs up to 12 weeks is feasible with acceptable recovery rates and low hemolysis (0.6%).
  • Normal 2,3-DPG concentrations can be maintained for two weeks under these storage conditions.