Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoxia rapidly activates HIF-3alpha mRNA expression.

Marc Heidbreder1, Frederike Fröhlich, Olaf Jöhren

  • 1Institute of Experimental and Clinical Pharmacology and Toxicology, University Luebeck, Ratzeburger Allee 160, D-23538 Luebeck, Germany. heidbred@medinf.mu-luebeck.de

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|June 26, 2003
PubMed
Summary

Hypoxia-inducible factor 3-alpha (HIF-3alpha) mRNA uniquely increases with hypoxia, unlike other HIFs. This suggests HIF-3alpha plays a rapid role in cellular protection against hypoxic damage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Ex-vivo</i> culture of human hypertrophic cardiomyopathy hearts: Functional and metabolic changes during long-term culture.

iScience·2026
Same author

Identification of an Angiotensin-(1-7)-Producing fusion protein in the brain of transgenic rats Reveals a hypotensive effect mediated through modulation of the GABA-nNOS-NO pathway and highlighting Astrocyte-Neuron crosstalk.

Neuroscience·2026
Same author

Translational Insights Into Myocardial Deformation and Fibrosis in Hypertrophic Cardiomyopathy Using Diffusion Tensor MRI.

JACC. Advances·2025
Same author

Regulation of hedonic feeding rhythms by circadian clocks in leptin-receptive neurons.

Molecular metabolism·2025
Same author

Thyroid hormone receptor beta (THRB) dependent regulation of diurnal hepatic lipid metabolism in adult male mice.

npj metabolic health and disease..·2025
Same author

Dexrazoxane protects against doxorubicin-induced cardiotoxicity in susceptible human living myocardial slices: A proof-of-concept study.

British journal of pharmacology·2025

Area of Science:

  • Molecular Biology
  • Physiology
  • Cellular Biology

Background:

  • The roles of HIF-1alpha and HIF-1beta in hypoxia are known, but HIF-2alpha and HIF-3alpha organ distribution and regulation remain unclear.
  • Understanding HIF subunit behavior is crucial for cellular response to low oxygen environments.

Purpose of the Study:

  • To investigate the organ distribution and transcriptional regulation of all hypoxia-inducible factor (HIF) subunits (HIF-1alpha, HIF-1beta, HIF-2alpha, HIF-3alpha) under systemic hypoxia in rats.
  • To examine the mRNA levels of HIF target genes, erythropoietin (EPO) and glucose-transporter 1 (GLUT1), in response to hypoxia.

Main Methods:

  • Quantitative real-time RT-PCR was used to measure mRNA levels of HIF subunits and target genes (EPO, GLUT1) in various rat organs.
  • Immunoblotting was employed to determine HIF subunit protein levels in brain and lung tissue.

Related Experiment Videos

Main Results:

  • HIF-1alpha, -1beta, and -2alpha mRNA levels were unaffected by hypoxia, while HIF-3alpha mRNA significantly increased in all examined organs after 2 hours.
  • EPO and GLUT1 mRNA levels rose significantly in cortex, heart, liver, and kidney after 2 hours of hypoxia, indicating HIF system activation.
  • HIF subunit protein levels increased with hypoxia duration in brain and lung.

Conclusions:

  • HIF-3alpha exhibits unique transcriptional induction under hypoxia, differentiating it from other HIF subunits.
  • HIF-3alpha may function as a rapid-response component of the HIF system, contributing to protection against hypoxic injury.