Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods to study p53-repressed promoters.

Patrick Dumont1, Anthony Della Pietra, Maureen E Murphy

  • 1Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 26, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The biology of hypomorphic TP53 variants and implications for clinical management.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

A phylogeny-guided framework for decoding mechanisms of human endogenous retrovirus regulation in health and disease.

bioRxiv : the preprint server for biology·2026
Same author

Variation at the R181 residue of p53 confers loss of p53 DNA binding cooperativity with the retention of mitochondrial-associated apoptosis.

Molecular cancer research : MCR·2026
Same author

A Personalized Therapeutic Approach for Liver Cancers Expressing the African-Centric P47S Variant of TP53.

Molecular cancer research : MCR·2026
Same author

Club cell secretory protein (CCSP) serum concentration is a prognostic factor after surgical resection of localized non-small cell lung cancer in patients in the IFCT-0302 trial.

Lung cancer (Amsterdam, Netherlands)·2026
Same author

A K27-linked Ubiquitin Checkpoint Controls NOTCH Homeostasis.

bioRxiv : the preprint server for biology·2025

The p53 tumor suppressor protein represses gene transcription. New methods, including the McKay assay and chromatin immunoprecipitation (ChIP), help define how p53 represses genes, aiding research into cell growth arrest and apoptosis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • The p53 protein is a crucial tumor suppressor.
  • p53 acts as both a transcriptional activator and repressor.
  • Understanding p53's repressive function is vital for cancer biology.

Purpose of the Study:

  • To explore alternative methods for studying p53's transcriptional repression.
  • To identify sequence elements mediating p53-mediated repression.
  • To clarify the mechanism of p53's repressive activity.

Main Methods:

  • Utilizing the McKay (immunobinding) assay for in vitro DNA binding.
  • Employing chromatin immunoprecipitation (ChIP) for in vivo binding analysis.
  • Comparing these methods to traditional transient transfection assays.

Related Experiment Videos

Main Results:

  • Alternative assays provide clearer insights into p53's repressive function.
  • McKay and ChIP assays facilitate the definition of DNA elements involved in repression.
  • These methods overcome limitations of overexpression-based assays.

Conclusions:

  • New in vitro and in vivo assays are essential for defining p53's transcriptional repression mechanism.
  • Accurate understanding of p53 repression aids in studying its role in apoptosis and growth arrest.
  • Further research using these methods will elucidate p53's tumor suppressor functions.