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Reconstitution and assay of biogenic membrane-derived phospholipid flippase activity in proteoliposomes

Sathyanarayana N Gummadi1, Sigrún Hrafnsdóttir, Jane Walent

  • 1Department of Chemical Engineering, Indian Institute of Technology-Madras, Chennai, India.

Methods in Molecular Biology (Clifton, N.J.)
|June 26, 2003
PubMed
Abstract

No abstract available in PubMed .

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Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
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Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...