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Updated: Jul 20, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities
P A Rochelle1, J C Fry, R J Parkes
1School of Pure and Applied Biology, University of Wales College of Cardiff, UK.
Abstract:
A protocol was devised which permitted the extraction of DNA from deep marine sediments up to 503 m below the sea floor. These sediments have been laid down over the last 3 million years. 16S rRNA gene sequences were amplified from the DNA by the polymerase chain reaction. The details of the successful extraction and polymerase chain reaction methodology varied between samples from different depths. This emphasizes the attention to detail required to allow the diversity of bacteria in these deep sediments to be studied.

