Expression of the CD15 epitope in the human magnocellular basal forebrain system

The distribution of the carbohydrate epitope 3-fucosyl-N-acetyl-lactosamine (CD15) has been immunocytochemically evaluated in coronal paraffin sections through the magnocellular basal forebrain system--the nucleus basalis of Meynert, the nucleus of the diagonal band of Broca and the medial septal nucleus--of 202 human brains. The brains derived from differently aged controls (n = 54) and from patients suffering from organic brain diseases (n = 129) or psychiatric disorders (n = 19). In 30 cases dementia was clinically diagnosed. CD15 first appeared around birth when it became localized on singular astrocytes. The astrocyte number and process density steadily increased, and at approximately 12 years the typical adult-type pattern was acquired. Considerable variations in the expression patterns were noted with regard to the astrocyte number, the intensity in immunostaining and the process relations of CD15-positive astrocytes with the magnocellular neurons. In the light of these variations, and of conflicting additional changes in other areas of most diseased brains, it was difficult to correlate different intensities and patterns to specific diseases. The results, however, provide evidence for an increase in CD15 expression and in process network density of astrocytes in the lateral part of the nucleus basalis of Meynert in cases of Huntington's disease.