Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flip the tip: an automated, high quality, cost-effective patch clamp screen.

Albrecht Lepple-Wienhues1, Klaus Ferlinz, Achim Seeger

  • 1Flyion GmbH, Tübingen, Waldhäuserstr. 64, D-72076, Tübingen, Germany. info@flyion.com

Receptors & Channels
|June 27, 2003
PubMed
Summary

A novel automated patch clamp technology achieves high-rate gigaseals and whole-cell recordings. This method ensures stable recordings and high throughput for drug discovery and electrophysiology research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artifact Reduction in the Diagnosis of Vasospasm in Computed Tomographic Perfusion: Potential of Iterative Metal Artifact Reduction.

Journal of computer assisted tomography·2019
Same author

Hypercapnic BOLD MRI compared to H<sub>2</sub><sup>15</sup>O PET/CT for the hemodynamic evaluation of patients with Moyamoya Disease.

NeuroImage. Clinical·2019
Same author

Assessment of uveal melanomas using advanced diffusion-weighted imaging techniques: value of reduced field of view DWI ("zoomed DWI") and readout-segmented DWI (RESOLVE).

Acta radiologica (Stockholm, Sweden : 1987)·2018
Same author

Advanced diffusion-weighted imaging in patients with optic neuritis deficit - value of reduced field of view DWI and readout-segmented DWI.

The neuroradiology journal·2018
Same author

Impact of Papillary Muscle Infarction on Ischemic Mitral Regurgitation Assessed by Magnetic Resonance Imaging.

RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2017
Same author

Morphologic and Clinical Outcome of Intracranial Aneurysms after Treatment Using Flow Diverter Devices: Mid-Term Follow-Up.

Radiology research and practice·2016

Area of Science:

  • Electrophysiology
  • Biotechnology

Background:

  • The development of automated patch clamp systems is crucial for high-throughput screening.
  • Achieving stable gigaseals and whole-cell access remains a challenge in automated systems.

Purpose of the Study:

  • To present a novel automated technology for high-rate gigaseal formation and whole-cell recordings.
  • To demonstrate the system's capability for stable, long-term electrophysiological recordings across various cell types.

Main Methods:

  • Automated cell suspension flushing towards glass micropipette tips.
  • Automated seal formation, whole-cell break-in, and pipette/liquid handling.
  • Utilizing pulled micropipettes as a low-cost, high-quality gigaseal substrate.

Main Results:

Related Experiment Videos

  • High-rate production of true gigaseals and whole-cell preparations.
  • Extremely stable seals and access resistance ensuring high recording quality.
  • Demonstrated long-term stability, voltage clamp quality, and pM compound block in various cell types.

Conclusions:

  • The novel automated patch clamp technology enables efficient and high-quality electrophysiological recordings.
  • The system offers a flexible, cost-effective solution with minimized consumables for maximal throughput.
  • This technology advances automated electrophysiology for drug discovery and research applications.