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Placental protein 14 regulates selective B cell responses
Einat Yaniv1, Zipora Borovsky, Galit Mishan-Eisenberg
1Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Jerusalem, Israel.
Cellular Immunology
|June 27, 2003
Summary
Placental protein 14 (PP14) inhibits B cell activation, affecting proliferation and IgM secretion. This immunosuppressive glycoprotein also impacts T cells, influencing both cellular and humoral immune responses.
Area of Science:
- Immunology
- Glycoprotein Function
- Cellular Signaling
Background:
- Placental protein 14 (PP14) is a lipocalin family glycoprotein.
- PP14 is known to negatively regulate T cell receptor-mediated activation.
Purpose of the Study:
- To investigate the role of PP14 in regulating B cell activation.
- To understand PP14's impact on immune responses.
Main Methods:
- Investigated PP14 effects on B cell proliferation, IgM secretion, and surface molecule expression (MHC class II, CD69, CD86).
- Stimulated B cells using anti-IgM, protein kinase C activators with Ca(2+) ionophore.
- Compared PP14's effects to anti-CD19 mAb ligation.
Main Results:
- PP14 inhibited B cell proliferation, IgM secretion, and MHC class II expression.
- Effects were independent of anti-IgM concentration, T cells, IL-4, and early B cell activation events.
- PP14's inhibitory action mimicked CD19 ligation.
Conclusions:
- PP14 acts as a soluble regulatory factor impacting both T and B cells.
- PP14 influences cellular and humoral immune responses via carbohydrate-dependent interactions.